ELISA 17-ketosteroids (17-KS)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:17-KS Alternative Names:N/A Application:ELISA Range:3.125-200 ng/mL Sensitivity:1.25 ng/mL Intra-AssayCV:?7.1% Inter-AssayCV:?8.5% Recovery:0.96 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate 17-KS in samples. An antibody specific for 17-KS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any17-KS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for 17-KS is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 17-KS bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:A 17-ketosteroid is a steroid that has a ketone (C=O) functional group at the C17 position (in the upper right corner of most diagrams.) These compound are products of metabolization of testosterone in most tissues such as liver. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).