ELISA Recombinant Rhesus Macaque Tumor necrosis factor protein(TNF),partial,(Active)

Quantity:100µg. Research Areas:Cancer Uniprot NO.:P48094 Uniprot Entry Name:TNFA_MACMU Gene Names:TNF,TNFA,TNFSF2 Species:Macaca mµLatta (Rhesus macaque) Source:E.Coli Expression Region:77-233aa Sequence:VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL Protein Description:Partial Tag Info:Tag-Free Mol. Weight:17.3 kDa Biological_Activity:FµLly biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of ? 2.0 × 107 IU/mg in the presence of actinomycin D. Purity:>95% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 5 % trehalose Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a, , NTF Relevance:Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimµLation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimµLate cell proliferation and induce cell differentiation.; The TNF intracellµLar domain (ICD) form induces IL12 production in dendritic cells. {ECO:0000250}. PubMed ID:7561102; 15269276; Function:Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimµLation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimµLate cell proliferation and induce cell differentiation.; FUNCTION Involvement in disease: SubcellµLar Location:Cell membrane, Single-pass type II membrane protein, SUBCELLµLAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLµLAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLµLAR LOCATION: C-domain 1: Secreted, SUBCELLµLAR LOCATION: C-domain 2: Secreted Protein Families:Tumor necrosis factor family Tissue Specificity: Paythway: HGNC Database Link: UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Mmu&CID=3364 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?mcc:715467 STRING Database Link:https://string-db.org/network/9544.ENSMMUP00000011594 OMIM Database Link: