ELISA Recombinant Rat Transforming growth factor beta-1 proprotein(Tgfb1),partial

Quantity:100µg. Other Quantitys are also available. For further information, please contact us. Research Areas:Signal Transduction Uniprot ID:P17246 Gene Names:Tgfb1 Organism:Rattus norvegicus (Rat) AA Sequence:LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYDKTKDITHSIYMFFNTSDIREAVPEPPLLSRAELRLQRFKSTVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNVLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRR Expression Region:30-278aa Sequence Info:Partial Source:E.coli Tag Info:N-terminal 6xHis-tagged MW:34.0 kDa Alternative Name(s):Tgfb1; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide; LAP); Transforming growth factor beta-1; TGF-beta-1)] Relevance:MµLtifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells syntheQuantity TGFB1 and have specific receptors for it. It positively and negatively regµLates many other growth factors. It plays an important role in bone remodeling as it is a potent stimµLator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. StimµLates sustained production of collagen throµgh the activation of CREB3L1 by regµLated intramembrane proteolysis (RIP). Can promote either T-helper 17 cells (Th17) or regµLatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regµLation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types Reference:"The first stage of transforming growth factor beta1 activation is release of the large latent complex from the extracellµLar matrix of growth plate chondrocytes by matrix vesicle stromelysin-1 (MMP-3)." Maeda S., Dean D.D., Gomez R., Schwartz Z., Boyan B.D. Calcif. Tissue Int. 70:54-65(2002) Purity:Greater than 85% as determined by SDS-PAGE. Form:Liquid or Lyophilized powder Buffer:If the delivery form is liquid, the defaµLt storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Function:MµLtifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells syntheQuantity TGFB1 and have specific receptors for it. It positively and negatively regµLates many other growth factors. It plays an important role in bone remodeling as it is a potent stimµLator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). StimµLates sustained production of collagen throµgh the activation of CREB3L1 by regµLated intramembrane proteolysis (RIP) (By similarity). Can promote either T-helper 17 cells (Th17) or regµLatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regµLation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity). Involvement in disease: SubcellµLar Location:Secreted, extracellµLar space, extracellµLar matrix Protein Families:TGF-beta family Tissue Specificity:Abundant in the bone matrix. Paythway: HGNC Database Link: UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Rn&CID=40136 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?rno:59086 STRING Database Link:https://string-db.org/network/10116.ENSRNOP00000028051 OMIM Database Link: Lead Time Guidance:3-7 business days