ELISA Recombinant Rhodobacter sphaeroides Modification methylase RsrI(rsrIM)

Quantity: 200µg. Other Quantitys are also available. Please Inquire. In Stock: No Lead time: 10-20 working days Research Topic: Others Uniprot ID: P14751 Gene Names: rsrIM Organism: Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) AA Sequence: MANRSHHNAGHRAMNALRKSGQKHSSESQLGSSEIGTTRHVYDVCDCLDTLAKLPDDSVQLIICDPPYNImLADWDDHMDYIGWAKRWLAEAERVLSPTGSIAIFGGLQYQGEAGSGDLISIISHMRQNSKmLLANLIIWNYPNGMSAQRFFANRHEEIAWFAKTKKYFFDLDAVREPYDEETKAAYMKDKRLNPESVEKGRNPTNVWRMSRLNGNSLERVGHPTQKPAAVIERLVRALSHPGSTVLDFFAGSGVTARVAIQEGRNSICTDAAPVFKEYYQKQLTFLQDDGLIDKARSYEIVEGAANFGAALQRGDVAS Expression Region: 1-319aa Sequence Info: FµLl Length Source: E.coli Tag Info: N-terminal 6xHis-SUMO-tagged MW: 51.7 kDa Alternative Name(s): Adenine-specific methyltransferase RsrI Relevance: This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the RsrI endonuclease. Reference: Purification, cloning and sequence analysis of RsrI DNA methyltransferase lack of homology between two enzymes, RsrI and EcoRI, that methylate the same nucleotide in identical recognition sequences.Kaszubska W., Aiken C., O'Connor D., Gumport R.I., Stephenson F.H., Greene P.J.Nucleic Acids Res. 17:10403-10425(1989) Purity: Greater than 90% as determined by SDS-PAGE. Storage Buffer: Tris-based buffer，50% glycerol Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.