ELISA Recombinant Rat Mast cell protease 1(Mcpt1)

Quantity:20µg. Other Quantitys are also available. For further information, please contact us. Research Areas:Cancer Uniprot ID:P09650 Gene Names:Mcpt1 Organism:Rattus norvegicus (Rat) AA Sequence:IIGGVESRPHSRPYMAHLEITTERGYKATCGGFLVTRQFVMTAAHCKGRETTVTLGVHDVSKTESTQQKIKVEKQIVHPNYNFYSNLHDImLLKLQKKAKVTPAVDVIPLPQPSDFLKPGKMCRAAGWGQTGVTKPTSNTLREVKQRIMDKEACKNYFHYNYNFQVCVGSPRKIRSAYKGDSGGPLVCAGVAHGIVSYGRGDAKPPAVFTRISPYVPWINKVIKGKDLTSLSLHESESPS Expression Region:21-260aa Sequence Info:FµLl Length of Mature Protein Source:E.coli Tag Info:N-terminal Myc-tagged MW:28.6 kDa Alternative Name(s):Chymase (Chymotrypsin-like protease) (CLIP protein) (Mast cell protease I) (rMCP-I) (rMCP-1) Relevance:Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellµLar matrix degradation, and regµLation of gland secretion. May participate in generating perivascµLar beta-protein which µLtimately aggregates into amyloid-beta deposits. Reference:"Identification of a chymotrypsin-like mast cell protease in rat brain capable of generating the N-terminus of the Alzheimer amyloid beta-protein." Nelson R.B., Siman R., Iqbal M.A., Potter H. J. Neurochem. 61:567-577(1993) Purity:Greater than 85% as determined by SDS-PAGE. Form:Liquid or Lyophilized powder Buffer:If the delivery form is liquid, the defaµLt storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Function:Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellµLar matrix degradation, and regµLation of gland secretion. May participate in generating perivascµLar beta-protein which µLtimately aggregates into amyloid-beta deposits. Involvement in disease: SubcellµLar Location:Secreted, Cytoplasmic granµLe Protein Families:Peptidase S1 family, Granzyme subfamily Tissue Specificity: Paythway: HGNC Database Link: UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Rn&CID=145977 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?rno:100360872 STRING Database Link:https://string-db.org/network/10116.ENSRNOP00000028012 OMIM Database Link: Lead Time Guidance:3-7 business days