ELISA Recombinant Streptomyces caespitosus Modification methylase ScaI(scaIM)

Quantity: 200µg. Other Quantitys are also available. Please Inquire. In Stock: No Lead time: 10-20 working days Research Topic: Others Uniprot ID: O52692 Gene Names: scaIM Organism: Streptomyces caespitosus AA Sequence: MSGRDFGYVIQSSAALWNRLSTFSQRGKALDTRLADIKKALGKPYYETSDVLLYHGDSLELLKSMPQQIFDLTVTSPPYNIGKEYEGVLSIEEYISWCETWMSRVHRATSAGGAFWLNVGYVPVPNQGKAVPIPYLLWDKSPFYMIQEVVWNYGAGVASRKSFSPRNEKFLWYVRDPLNYYFDLDSVRDPNVKYPNQKKNGKLKCNPLGKNPTDVWQFPKVTSGAKRSSVERTAHPAQFPSAVIERVIKACSPSDGVILDPFLGSGTTSLTARKQGRCSVGIEIREDYLDIAVGRLEAEAQSLF Expression Region: 1-304aa Sequence Info: FµLl Length Source: E.coli Tag Info: N-terminal 6xHis-SUMO-tagged MW: 50.2 kDa Alternative Name(s): N-4 cytosine-specific methyltransferase ScaI Relevance: This methylase recognizes the double-stranded sequence AGTACT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the ScaI endonuclease. Reference: Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli.Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. IIMol. Gen. Genet. 260:226-231(1998) Purity: Greater than 90% as determined by SDS-PAGE. Storage Buffer: Tris-based buffer，50% glycerol Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.