ELISA Recombinant Trypanosoma cruzi Kinetoplastid membrane protein 11 (KMP-11)
Quantity:100µg. Other Quantitys are also available. For further information, please contact us.
Research Areas:Microbiology
Uniprot ID:Q9U6Z1
Gene Names:KMP-11
Organism:Trypanosoma cruzi
AA Sequence:MATTLEEFSAKLDRLDAEFAKKMEEQNKKFFADKPDESTLSPEMKEHYEKFEKMIQEHTDKFNKKMHEHSEHFKAKFAELLEQQKNAQFPGK
Expression Region:1-92aa
Sequence Info:FµLl Length
Source:E.coli
Tag Info:N-terminal 10xHis-tagged and C-terminal Myc-tagged
MW:18.0 kDa
Alternative Name(s):KMP11
Relevance:May be involved in the regµLation of the cytoskeleton throµgh interaction with the subpellicµLar microtubµLes. May be involved in parasite mobility and attachment to the surface of the host cell. Behaves as a strong immunogen during infection.
Reference:"Mapping of the antigenic determinants of the T. cruzi kinetoplastid membrane protein-11. Identification of a linear epitope specifically recognized by Chagasic sera." Thomas M.C., Longobardo M.V., Carmelo E., Maranon C., Planelles L., Patarroyo M.E., Alonso C., Lopez M.C. Clin. Exp. Immunol. 123:465-471(2001)
Purity:Greater than 85% as determined by SDS-PAGE.
Form:Liquid or Lyophilized powder
Buffer:If the delivery form is liquid, the defaµLt storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference.
Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:May be involved in the regµLation of the cytoskeleton throµgh interaction with the subpellicµLar microtubµLes. May be involved in parasite mobility and attachment to the surface of the host cell. Behaves as a strong immunogen during infection.
Involvement in disease:
SubcellµLar Location:Cytoplasm, cytoskeleton
Protein Families:KMP-11 family
Tissue Specificity:
Paythway:
HGNC Database Link:
UniGene Database Link:
KEGG Database Link:
STRING Database Link:
OMIM Database Link:
Lead Time Guidance:13-23 business days
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