ELISA PRKN anti-

Quantity :50µg Clone Number: Aliases:AR JP antibody; E3 ubiquitin ligase antibody; E3 ubiquitin protein ligase parkin antibody; E3 ubiquitin-protein ligase parkin antibody; FRA6E antibody; LPRS 2 antibody; LPRS2 antibody; PARK 2 antibody; Park2 antibody; Parkin 2 antibody; Parkinson disease (autosomal recessive juvenile) 2 antibody; Parkinson disease (autosomal recessive; juvenile) 2; parkin antibody; Parkinson disease protein 2 antibody; Parkinson juvenile disease protein 2 antibody; Parkinson protein 2 E3 ubiquitin protein ligase antibody; Parkinson protein 2; E3 ubiquitin protein ligase (parkin) antibody; PDJ antibody; PRKN 2 antibody; PRKN antibody; PRKN2 antibody; PRKN2_ antibody; Ubiquitin E3 ligase PRKN antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:O60260 Immunogen:Recombinant E3 ubiquitin-protein ligase parkin protein (1-465AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:500 Background:Functions within a mµLtiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2. Mediates monoubiquitination as well as \\\'Lys-48\\\'-linked and \\\'Lys-63\\\'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating \\\'Lys-63\\\'-linked polyubiquitination of misfolded proteins such as PARK7: \\\'Lys-63\\\'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates \\\'Lys-63\\\'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regµLator of autophagy. Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by promoting the ubiquitination of mitochondrial proteins such as TOMM20, RHOT1/MIRO1 and USP30 (PubMed:24896179). Mediates \\\'Lys-48\\\'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in the regµLation of neuron death. Limits the production of reactive oxygen species (ROS). RegµLates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress. Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumµLation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:PRKN Research Areas:Neuroscience; Cell biology; Metabolism