ELISA PRKDC anti-

Quantity :100µL Clone Number: Aliases:DNA dependent protein kinase catalytic subunit antibody; DNA PK catalytic subunit antibody; DNA-dependent protein kinase catalytic subunit antibody; DNA-PK catalytic subunit antibody; DNA-PKcs antibody; DNAPK antibody; DNAPK catalytic subunit antibody; DNPK 1 antibody; DNPK1 antibody; Hyper radiosensitivity of murine scid mutation, complementing 1 antibody; Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody; HYRC 1 antibody; HYRC antibody; HYRC1 antibody; IMD26 antibody; p350 antibody; p460 antibody; PKRDC antibody; PRKDC antibody; PRKDC_ antibody; Protein Kinase DNA Activated Catalytic Polypeptide antibody; XRCC 7 antibody; XRCC7 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P78527 Immunogen:SyntheQuantityd peptide derived from DNA-PK. Raised in:Rabbit Reactivity:, Mouse Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 Background:Serine/threonine-protein kinase that acts as a molecµLar sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, sµggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modµLation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regµLating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely throµgh an indirect machanism. Interacts with CRY1 and CRY2; negatively regµLates CRY1 phosphorylation. Wesley D. Block, Nucleic Acids Res., Apr 2004; 32: 1967 - 1972. Katherine S. Pawelczak, Nucleic Acids Res., Jan 2005; 33: 152 - 161. Eun-Jung Park, Nucleic Acids Res., Dec 2003; 31: 6819 - 6827. Suisheng Zhang, Nucleic Acids Res., Jan 2004; 32: 1 - 10. Clonality:Polyclonal Isotype:IgG Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:PRKDC Research Areas:Epigenetics and Nuclear Signaling