ELISA EP300 anti-

Quantity :100µL Clone Number: Aliases:E1A associated protein p300 antibody; E1A binding protein p300 antibody; E1A-associated protein p300 antibody; EP300 antibody; EP300: E1A binding protein p300 antibody; EP300_ antibody; Histone acetyltransferase p300 antibody; KAT3B antibody; p300 HAT antibody; RSTS2 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q09472 Immunogen:SyntheQuantityd peptide derived from N-terminal of p300. Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB, IHC; WB:1:500-1:3000, IHC:1:50-1:100 Background:Functions as histone acetyltransferase and regµLates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regµLation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimµLates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 throµgh acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modµLation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. RegµLates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regµLated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Wuchao Yuan, J. Biol. Chem., Apr 1996; 271: 9009. E. Tory Manning, Mol. Cell. Biol., Jun 2001; 21: 3876 - 3887. Jiwen Li, Mol. Cell. Biol., Mar 2000; 20: 2031 - 2042. Gopalswamy Jayaraman, J. Biol. Chem., Jun 1999; 274: 17342. Clonality:Polyclonal Isotype:IgG Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:EP300 Research Areas:Epigenetics and Nuclear Signaling;Cancer;Metabolism;Stem cells