ELISA RPTOR anti-

Quantity :50µL Clone Number:1B10 Aliases:RegµLatory-associated protein of mTOR (Raptor) (p150 target of rapamycin (TOR)-scaffold protein), RPTOR, KIAA1303 RAPTOR Product Type:Recombinant Antibody Immunogen Species:Homo sapiens () UniProt ID:Q8N122 Immunogen:A syntheQuantityd peptide derived from Raptor Raised in: Reactivity: Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:50-1:200 Background:Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regµLates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimµLated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the RagµLator complex and the Rag GTPases. Activated mTORC1 up-regµLates protein synthesis by phosphorylating key regµLators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. Clonality:Monoclonal Isotype:Rabbit IgG Purification Method:Affinity-chromatography Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:RPTOR Research Areas:Epigenetics and Nuclear Signaling; Cell biology; Signal transduction