ELISA Recombinant Streptomyces actuosus Nosiheptide precursor(nosM)

Quantity: 200µg. Other Quantitys are also available. Please Inquire. In Stock: No Lead time: 22-32 working days Research Topic: Others Uniprot ID: C6FX52 Gene Names: nosM Organism: Streptomyces actuosus AA Sequence: MDAAHLSDLDIDALEISEFLDESRLEDSEVVAKVMSASCTTCECCCSCSS Expression Region: 1-50aa Sequence Info: FµLl Length Source: Yeast Tag Info: N-terminal GST-tagged MW: 32.4 kDa Alternative Name(s): Antibiotic 9671-RP1 Publication MµLthiomycin Relevance: Inhibits bacterial protein biosynthesis by binding to ribosomes. Specifically, binds to the complex of 23S rRNA and ribosomal protein L11 (RPLK) in the 50S ribosomal subunit. While allowing a weak binding of elongation factor G (EF-G) to the ribosome and subsequent GTP-hydrolysis, probably impairs conformational changes in both the ribosome and EF-G which are necessary for translocation. In vitro, inhibits Gram-positive bacteria S.aureus strain 209P (MIC=0.0009 µg/mL), S.aureus strain 133 (MIC=0.0019 µg/mL), S.aureus strain B3 (MIC=0.003 µg/mL), S.aureus strain Hb (MIC=0.003 µg/mL), M.citreus strain ATCC 8411 (MIC=0.0038 µg/mL), M.lysodeikticus strain ATCC 4698 (MIC=0.003 µg/mL), S.lutea strain ATCC 9341 (MIC=0.0011 µg/mL), S.faecalis strain ATCC 9790 (MIC=0.0007 µg/mL), S.viridans (MIC=0.0065 µg/mL), S.pyogenes hemolyticus strain Dig7 (MIC=0.00028 µg/mL), D.pneumoniae strain Til (MIC=0.00015 µg/mL), N.catrrhalis (MIC=0.0017 µg/mL), L.casei strain ATCC 6633 (MIC=0.003 µg/mL), B.cereus strain ATCC 6630 (MIC=0.0071 µg/mL) and various isolates of L.monocytogenes. In vitro, inhibits Gram-negative bacterium P.mµLtocida strain A125 (MIC=0.0024 µg/mL) but not M.smegmatis strain ATCC 6630, S.typhimurium, A.aerogenes strain ATCC 8308, P.vµLgaris, K.pneumoniae strain ATCC 10031, S.marcescens strain A476, P.aerµginosa strain Bass or B.bronchiseptica strain CN387. Does not inhibit Gram-negative bacterium E.coli strain ATCC 9637 but does inhibit purified ribosomes from E.coli. In vivo, has no systemic effect in mice infected with staphylococci or streptococci when applied orally or subcutaneously. Has a local effect in mice infected subcutaneously or intraperitoneally with staphylococci when applied immediately afterwards. Is not toxic to mice. Reference: "Mapping the binding site of thiopeptide antibiotics by proximity-induced covalent capture."Baumann S., Schoof S., Harkal S.D., Arndt H.D. J. Am. Chem. Soc. 130:5664-5666(2008) Purity: Greater than 90% as determined by SDS-PAGE. Storage Buffer: Tris-based buffer，50% glycerol Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.