ELISA TransDetect® In Situ Fluorescein TUNEL Cell Apoptosis Detection

Product Details TransDetect® In Situ Fluorescein TUNEL Cell Apoptosis Detection Kit provides a precise, simple and low-toxicity way to detect and quantify apoptotic cell death at single cell level in cells and tissues. TdT-mediated dUTP Nick-End Labeling (TUNEL) reaction preferentially labels DNA strand breaks generated during apoptosis with fluorescein-labeled dUTP. The fluorescein labeled DNA can be detected and quantified by fluorescence microscopy or flow cytometry. This kit can be used to detect apoptosis in paraffin-embedded tissue sections, cryopreserved tissue sections, cells cµLtured on chamber slides, cell smear and cell suspensions. Highlights • Extremely low toxicity, labeling solution is free of commonly used highly-toxic cacodylate. • High sensitivity, high specificity, optimal ratio of labeled and non-labeled substrates. • Simple, one-step labeling by mixing TdT and labeling solution. • Flexible, can be used in many assay systems. Storage TdT at -20℃ for one year, 1×Labeling Solution at -20℃ in dark for one year Shipping Dry ice (-70℃) Product Contents Component FA201-01 (25 rxns) FA201-02 (50 rxns) TdT 50 μl 100 μl 1×Labeling Solution 1.25 mL 2×1.25 mL Citations Li D Y , Shi X J , Li W , et al. Ischemic preconditioning and remote ischemic preconditioning provide combined protective effect against ischemia/reperfusion injury[J]. Life Sciences, 2016,150:76-80.