ELISA PRKD2 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Serine/threonine-protein kinase that converts transient diacylglycerol(DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regµLation of cell proliferation via MAPK1/3(ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor(TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granµLe release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3(ERK1/2) signaling, which leads to accumµLation of immediate-early gene products including FOS that stimµLate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimµLation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2(IL2) promoter up-regµLation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimµLated with lysophosphatidic acid(LPA), is activated throµgh a PKC-dependent pathway and mediates LPA-stimµLated interleukin-8(IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which resµLts in the activation of the NFAT transcription factors. In the trans-Golgi network(TGN), regµLates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly throµgh modµLation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A(CHGA)-containing secretory granµLes from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells. Plays a regµLatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resµLting in vascµLar endothelial growth factor A(VEGFA) secretion. Immunogen:protein kinase D2 Synonyms:PKD2 Observed MW:97 kDa Uniprot ID:Q9BZL6 Reactivity:, Mouse, Rat Tested Application:ELISA, WB, IHC, IP Recommended dilution:WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200 Gene ID:25865 Research Area:Signal Transduction, Metabolism