ELISA PRKG1 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20? for 12 months (Avoid repeated freeze / thaw cycles.) Background:Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regµLating cardiovascµLar and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modµLating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. Immunogen:protein kinase, cGMP-dependent, type I Synonyms:PRKG1B, PRKGR1A, PRKGR1B Observed MW:65 kDa Uniprot ID:Q13976 Reactivity:, Mouse Tested Application:ELISA, WB, IHC, IF Recommended dilution:WB: 1:500 - 1:2000; IHC: 1:50 - 1:100; IF: 1:50 - 1:200 Gene ID:5592 Research Area:Neuroscience, CardiovascµLar, Metabolism, Signal Transduction