ELISA RIPK2 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Serine/threonine/tyrosine kinase that plays an essential role in modµLation of innate and adaptive immune responses. Upon stimµLation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 throµgh CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 throµgh Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor(TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Immunogen:receptor-interacting serine-threonine kinase 2 Synonyms:CARDIAK, RICK, RIP2 Observed MW:46-55 kDa, 61-72 kDa Uniprot ID:O43353 Reactivity:, Mouse, Rat Tested Application:ELISA, WB Recommended dilution:WB: 1:500-1:2000 Gene ID:8767 Research Area:Immunology, Signal Transduction, Metabolism