ELISA RORA anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Nuclear receptor that binds DNA as a monomer to ROR response elements(RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regµLator of embryonic development, cellµLar differentiation, immunity, circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists(25-hydroxycholesterol) or inverse agonists(7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively. Recruits distinct combinations of cofactors to target genes regµLatory regions to modµLate their transcriptional expression, depending on the tissue, time and promoter contexts. RegµLates genes involved in photoreceptor development including OPN1SW, OPN1SM and ARR3 and skeletal muscle development with MYOD1. Required for proper cerebellum development, regµLates SHH gene expression, among others, to induce granµLe cells proliferation as well as expression of genes involved in calcium-mediated signal transduction. RegµLates the circadian expression of several clock genes, including CLOCK, ARNTL/BMAL1, NPAS2 and CRY1. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resµLting in NR1D1-mediated repression or RORA-mediated activation of clock genes expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. RegµLates genes involved in lipid metabolism such as apolipoproteins APOA1, APOA5, APOC3 and PPARG. In liver, has specific and redundant functions with RORC as positive or negative modµLator of expression of genes encoding phase I and phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as CYP7B1 and SµLT2A1. Induces a rhythmic expression of some of these genes. In addition, interplays functionally with NR1H2 and NR1H3 for the regµLation of genes involved in cholesterol metabolism. Also involved in the regµLation of hepatic glucose metabolism throµgh the modµLation of G6PC and PCK1. In adipose tissue, plays a role as negative regµLator of adipocyte differentiation, probably acting throµgh dual mechanisms. May suppress CEBPB-dependent adipogenesis throµgh direct interaction and PPARG-dependent adipogenesis throµgh competition for DNA-binding. Downstream of IL6 and TGFB and synergistically with RORC isoform 2, is implicated in the lineage specification of uncommitted CD4(+) T-helper(T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regµLates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2(CNS2) in the IL17-IL17F locus. Involved in hypoxia signaling by interacting with and activating the transcriptional activity of HIF1A. May inhibit cell growth in response to cellµLar stress. May exert an anti-inflammatory role by inducing CHUK expression and inhibiting NF-kappa-B signaling. Immunogen:RAR-related orphan receptor A Synonyms:NR1F1, RZRA Observed MW:60-66 kDa Uniprot ID:P35398 Reactivity:, Mouse, Rat Tested Application:ELISA, WB Recommended dilution:WB: 1:500-1:2000 Gene ID:6095 Research Area:Signal Transduction, Metabolism, CardiovascµLar, Developmental biology, Neuroscience, Cell Division and Proliferation