ELISA RPA3 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:As part of the heterotrimeric replication protein A complex(RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellµLar response to DNA damage(PubMed:9430682). In the cellµLar response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Throµgh recruitment of ATRIP activates the ATR kinase a master regµLator of the DNA damage response(PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair(PubMed:7697716). Plays also a role in base excision repair(BER), probably throµgh interaction with UNG(PubMed:9765279). Throµgh RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA(PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression throµgh S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but coµLd support DNA synthesis by polymerase delta in presence of PCNA and replication factor C(RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange(PubMed:19996105). Immunogen:replication protein A3, 14kDa Synonyms:REPA3, RPA14 Observed MW: Uniprot ID:P35244 Reactivity: Tested Application:ELISA Recommended dilution: Gene ID:6119 Research Area:Metabolism