EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric)

A convenient package of tools that allows the experimenter to colorimetrically detect if histone H3 is acetylated, and colorimetrically quantify the amount of the acetylated histone H3. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) is suitable for specifically measuring total histone H3 acetylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages: • Quick and efficient procedure, which can be finished within 2.5 hours. • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography. • Captures histone H3 acetylated at any lysine site with the detection limit as low 2 ng/well and detection range from 5 ng-2 ug/well of histone extracts. • The control is conveniently included for the quantification of the amount of acetylated histone H3. • Strip microplate format makes the assay flexible: manual or high throughput. • Simple, reliable, and consistent assay conditions.