EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

A complete set of optimized buffers and reagents to colorimetrically quantify N6-methyladenosine (m6A or 6mA) in RNA. It is suitable for a direct detection of m6A RNA methylation status using total RNA isolated from any species such as mammals, plants, fungi, bacteria, and viruses. The kit has the following advantages and features: • Colorimetric ELISA-like assay using an m6A antibody with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 3 hours and 45 minutes. • High sensitivity, of which the detection limit can be as low as 10 pg of m6A. • Unique binding solution allows RNA >70 nts to be tightly bound to the wells, which enables quantification of m6A from both mRNA and ncRNA such as tRNA, rRNA, and snRNA. • optimized antibody and enhancer solutions allow high specificity to m6A, with no cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample RNA. • Universal positive and negative controls are included, which are suitable for quantifying m6A from any species. • Strip-well microplate format makes the assay flexible for manual or high throughput analysis. • Simple, reliable, and consistent assay conditions.